ࡱ> lnkY RB\bjbj[[8t9 \9 \t t    8CD  X'(>A]&&&&&&&$\),J&immm&H'mX&m&:",c#v,R(# o&('0X'#R\,*,c#\,d,$c# mmmmmmm&&mmmX'mmmm,mmmmmmmmmt I : uirb/g~T[ zYef['Y~ zW,gOo`Course Information * zNxCourse Code BI411*f[eCredit Hours 64*f[RCredits 2* zTyCourse Name uirb/g~T[Comprehensive experiment for biotechnology z'`((Course Type)W{QRQ zc[aAudience uirb/gNN,gyuc(Language of Instruction)-Ne*_b|School u}Tyf[b/gf[bHQO zPrerequisite RP[uirf[ uirSf[ _uirf[ WV] zI{cYe^Instructor UO=Nf zQ@W(Course Webpage) HYPERLINK "http://cc.sjtu.edu.cn/G2S/site/preview" \l "/home/v?currentoc=8007" http://cc.sjtu.edu.cn/G2S/site/preview#/home/v?currentoc=8007 HYPERLINK "http://www.cnmooc.org/portal/session/bulletin/index/2620.mooc" http://www.cnmooc.org/portal/session/bulletin/index/2620.mooc* z{NDescription ,g z/fbT,gbuirb/gNN,gyuv[ z,e(WW{Qccuirb/gv[b v^ gv^] zW@xwƋ (WvsQWNNuiryf[txvzT^(uxvzvؚ~NMb0uirb/g/fu}Tyf[W-N8h_vyf[b/g [_N/fbVvNLuSV[uNR4ls^ [sTSORT荊_SU\g g ^gvW Vdkuirb/gReNMbvW{Q[NbVuirb/gNNvSU\wQ g͑vaIN0Vdk ,g zYef[Q[V~geuirb/g0ppN&qp݋U\_ Ǒ(uYe^cOB\!kN Tv[!jWW Sb Tˆ}vh}SOvg^TvRt[ 0 Nn'`grSOWVibXS^RRg 0 Nn~|SODNAmtDNA WVibXS^RRg T GFP~SSMQupSRg Nf[uc[yvv~T NR~U\_[vb__[b[0 Te0N[[Yef[Kbk 8T_YecSN Ne9ei8hċ0Oe_egcؚf[uv[yg'`0[؏^zN 0[9eۏ^R?| 00gwSI{ SeNf[uvR`S[S v^\OQv^vte Ǐُ7hNev9eۏ f[uyg'`cؚN Rg0vtQ_NSmSN [bS㉳QvR_0RNR[cؚ0* z{NDescription This is an experimental course majoring in biotechnology for undergraduates in our school. It aims to cultivate senior talents who master the experimental skills of biotechnology and have relevant basic engineering knowledge, and can engage in the theoretical research and application research of biological science in related fields.Biotechnology is the core science and technology in the field of life science, and it is also the most promising field for China to catch up with the productivity level of the developed countries in the world and achieve late-development advantages and leapfrog development. Therefore, the cultivation of innovative talents of biotechnology is of great significance for the development of China.As a result, this course teaching the latest biotechnology, hot topic and focus topic, to adopt the teachers level different modules, including "the construction of a fusion protein expression vector and enzyme identification", "important sex chromosome gene amplification and sequence analysis", "anthropogenic mitochondrial DNA (mtDNA) gene amplification and sequence analysis" and "GFP purification and western blot analysis, the combination of complete the experiment in the form of grouping could begin.At the same time, we should enrich the experimental teaching methods, attract the professors to participate, and constantly reform the assessment methods to improve the students' enthusiasm for experiment.Experiment has also established the experimental suggestion book heroes, such as questionnaire, understand the student's dynamic and feedback in time, and make correspondi "VZ\dh  Ÿyod]X h]m+o( hDVh%OhDVh:B* phPh{qwB* o(phP h{qwh{qwhDVh:RHZo(hDVh0B*o(phhDVh:RHZh{qwh{qwRHZ hDVh:hDVh:o(hDVhGB*o(ph hDVh hDVh o( h h h h 5CJ aJ h]m+5CJ aJ o(h{qwh{qw5CJ aJ o( "XZfbVVMV $Ifgd), $$Ifa$gdDVykd$$Iflf;$&  t 0&644 lap ytDV $$Ifa$gdDV WD`gd: $YD2a$gdDV $Ifgd), $$Ifa$gdDV 6-- $Ifgd),kd$$Ifl/ֈ;L $f  t0&644 laytDV     n p r z |   " $ & ( 0 2 B D T X ` b ~ λλγΦλΒ΁λyλλh{qwh{qwo(hDVh0B*o(phh{qw h{qwo(hDVh}o( hDVhilhDVhilo( h]m+o(h@h{qwo(hDVh o(h{qwh{qwB* phP hDVh hDVh B* phPh@h{qwCJOJPJQJh@h{qwCJOJPJQJo(/    p t~kdm$$Ifl40;$f ^ t0&644 laytDV $$Ifa$gdDVp r | tth $$Ifa$gd]m+ $$Ifa$gdDV~kd$$Ifl40;$ f ^ t0&644 laytDV vvj $$Ifa$gd]m+ $$Ifa$gdDV}kd$$Ifl0;$f ^ t0&644 laytDV  $ xl` $$Ifa$gd]m+ $$Ifa$gdDV $$Ifa$gdDV{kd-$$Ifl0;$f ^ t0&644 laytDV$ & 2 D V xxl $$Ifa$gd]m+ $$Ifa$gdDV{kd$$Ifl0;$f ^ t0&644 laytDVV X b xxl $$Ifa$gd]m+ $$Ifa$gdDV{kdI$$Ifl0;$f ^ t0&644 laytDV     $ & ( * <>@BDHLfh߼{qhDVh RHZo(hDVh4z"o(hDVhaTVB*o(ph h{qwh%OhDVhc:B* phPh{qwPJaJhc:0JPJaJhc:PJaJjhc:PJUaJ hDVhCihhDVhCiho(hDVh}o( hDVh hDVh o(h{qw h{qwo(h{qwh{qwo('  ( @xxxxxll $$Ifa$gd{qw $$Ifa$gdDV{kd$$Ifl0;$f ^ t0&644 laytDV@BhF[O@1@$IfWD`gdY$IfWD`gduK $$Ifa$gdDVkde$$Ifl\; $f  /I t0&6 44 laytDVh$>@89999999&:(:x::::,;6;>;@;B;D;\;^;`;~;;;;;;;;;;߼߲ߙzmhDVhT B*o(ph h6heehPYhPYo(hPYhBNo(hBNhBNo( h5o(hDVhtB*o(ph hDVhT hDVhT o(UhYhDVh RHZo(hDVh o(hDVhaTVB*o(ph hDVh hYhYB* o(phPhuKhuKB* o(phP*@D9vjXX$i$IfWD2`ia$gd{ $$Ifa$gdY $$Ifa$gdDV}kd$$Ifl0;$f ^ t0&644 laytDVng adjustment, through continuous improvement, so students have improved since the enthusiasm, to analyze and discuss the problem, I also interested experiment skills and to improve the ability to solve problems effectively. zYef['Y~course syllabus *f[`Nvh(Learning Outcomes)1Nv^ccsNuirb/gSvQyrp Ƌuirb/g[N{|u;mNS>yOuN&^egvSiA5.4-5.602Ǐ zf[`N W{Qf[uwQ guirb/gvƉ҉ ㉳Q[ETs[vRB2, B303W{Qf[urz[vR v^:NۏeQyx[[z)R_U\[`Y[W@xB8,B90& & *Yef[Q[0ۏ^[cSBl(Class Schedule& Requirements)Yef[Q[f[eYef[e_\ONSBlW,gBlge_Tˆ}vh}SOvg^TvRt[32[+PPTGlb[b[S[U_0Rg[b[S[bJTPPTGlbJTNn'`grSOWVibXS^RRg32[+PPTGlb[b[S[U_0N`[b[S[bJTPPTGlbJTNn~|SODNAmtDNA WVibXS^RRg32[+PPTGlb[b[S[U_0N`[b[S[bJTPPTGlbJTGFP~SSMQupSRg32[+PPTGlb[b[S[U_0N`[b[S[bJTPPTGlbJT999y $IfgdCih}kd$$Ifla0;$f ^ t0&644 laytDV99(::::;@;ypppg $Ifgd6 $Ifgd5 $$Ifa$gdDVykdF$$Ifl-;$&  t 0&644 lap ytDV@;B;^;~;;;;;;;;ssseeeeZe $Ifgd),K$$$Ifa$gdDVK$$d4$Ifa$gdDV}kd$$Ifl)0;$f ^ t0&644 laytDV ;;;;;;;;;;;;<<<<<<<<< <&<,<2<6<@<B<F<P<\<^<`<~<<<<<<<<<<<<<<<<<<<<<<<<==hjh6aJ!hjh65OJQJ\aJo( hDVh6hR*Bh6o( hR*Bh6hR*Bh6aJ!hR*Bh65OJQJ\aJo( hDVh%OhDVh}ho(hDVhT o( hDVhT :;;;<<4&&&$$Ifa$gd6K$kd{$IfK$L$lֈae06Kz t0e644 laytDV<*<4<H<^<$$Ifa$gd6K$ $$Ifa$gd6^<`<<<<2$$$$$Ifa$gd6K$kd> $IfK$L$lֈae06Kz t0e644 laytrr2<<<<<$$Ifa$gd6K$ $$Ifa$gd6<<==0=2$$$$$Ifa$gd6K$kd $IfK$L$l+ֈae06Kz t0e644 laytrr2== ="=,=0=2=4=6=:=<=B=D=F=J=N=P=R=T=^=`=f=n=|=~==========================>ZZ$Z&Z*Z8Z:Z@ZFZHZJZLZNZhDVh0B*o(ph hDVhz hDVhT hjh6o(Uhjh6aJ!hjh65OJQJ\aJo( hDVh6 hR*Bh6hR*Bh6o( hjh6?0=F=R=f=|=$$Ifa$gd6K$ $$Ifa$gd6|=~====2$$$$$Ifa$gd6K$kd $IfK$L$l1ֈae06Kz t0e644 laytrr2=====$$Ifa$gd6K$ $$Ifa$gd6==>ZZ2$$$$$Ifa$gd6K$kd $IfK$L$l*ֈae06Kz t0e644 laytrr2& & YlMR3*N[:Nv^RsQ| f[uS3 1*8he_(Grading)b~gb [d\O40%+[bJT30%+[20%+[`N0[QY10%*YePgbSDe(Textbooks & Other Materials) 0RP[uirf[N~ހuirf[ 0 Y[Q -NVgNQHr>y 2010t^,{1Hr 978-7-5038-5774-4vQ[More YlNotes Ylf1&^*Q[:N_kXy02 z{NW[pe:N300-500W[ z'Y~NhnZiYef[[c:N[ W[peNP0ZZZ Z Z$kdZ $IfK$L$l(ֈae06Kz t0e644 laytDV$$Ifa$gd6K$ ZZZZZZZ$$Ifa$gd6K$ZZHZJZ2)) $Ifgd),kd'$IfK$L$l8ֈae06Kz t0e644 laytDVJZLZXZlZZxxl $$Ifa$gdDV $$Ifa$gdDV{kd$$Ifl0;$f ^ t0&644 laytDVNZVZXZjZlZxZZZZZZ[[[[*[,[.[0[8[:[H[N[R[T[X[Z[|[~[[[[[[[[[[[[𼯣}}}pehDVhT B* phPhDVh6B* o(phPh6CJPJh6CJPJo(hSPh6CJPJo(h{y CJPJo(h6CJOJPJQJh6CJOJPJQJo(h{y CJOJPJQJo(hDVhT B*o(phh6h6B* o(phPhDVhCihB* o(phP hDVhT hDVhT o('ZZZ[~[vvj $$Ifa$gd{y $$Ifa$gdDV}kd$$Iflr0;$f ^ t0&644 laytDV~[[[[[vvv $$Ifa$gdDV}kd$$Ifl:0;$f ^ t0&644 laytDV[[[[[vvv $$Ifa$gdDV}kd$$Ifl 0;$f ^ t0&644 laytDV[[[[[[[[[[[[$\&\(\,\.\2\4\8\:\@\B\ļhxjhxU haTVh hSt hSto( h@o( h haTVh haTVo( h=o( hDVhT hDVhT B* phP[[[[&\*\,\0\2\6\8\<\vhhcacacacgd~WdpWD`gd= $8XDda$gdDV}kd8$$Ifl 0;$f ^ t0&644 laytDV <\>\@\B\dpWD`gd=6182P:p). A!"#$%S $$If!vh#v':V lf  t 0&6,5&ap ytDV$$If!vh#v#v#vb#vB#v #vU:V l/ t0&6,5f 55555aytDV$$If!vh#v#v!:V l4 t0&6+,5f 5^aytDV$$If!vh#v#v!:V l4 t0&6+5f 5^aytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v#v=#v:V l t0&6 ,5f 5 5/5I aytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v!:V la t0&6,5f 5^/ aytDV$$If!vh#v':V l-  t 0&6,5&ap ytDV$$If!vh#v#v!:V l) t0&6,5f 5^aytDV$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l t0e6550565K5z5ytDV$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l t0e6550565K5z5ytrr2$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l+ t0e6550565K5z5ytrr2$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l1 t0e6550565K5z5ytrr2$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l* t0e6550565K5z5ytrr2$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l( t0e6,550565K5z5ytDV$IfK$L$l!vh#v#v0#v6#vK#vz#v:V l8 t0e6,550565K5z5ytDV$$If!vh#v#v!:V l t0&6,5f 5^aytDV$$If!vh#v#v!:V lr t0&6,5f 5^aytDV$$If!vh#v#v!:V l: t0&6,5f 5^aytDV$$If!vh#v#v!:V l  t0&6,5f 5^aytDV$$If!vh#v#v!:V l  t0&6,5f 5^aytDVԁ;AlK@y^ԁ;AlK@y^ԁ;AlK@y^ԁ;AlK@y^ s666666666vvvvvvvvv666666>6666666666666666666666666666666666666666666666666hH6666666666666666666666666666666666666666666666666666666666666666662 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p8XV~ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ OJPJQJ_HmH nHsH tHJ`J )cke $1$a$ CJKH_HaJmH nHsH tH$A $ ؞k=W[SOBi@B 0nfh*B*S*Y(phDbD ~W0u w9r G$Pa$CJaJ./q. ~W0u w CharCJaJ: : ~W0u9r G$a$CJaJ./. ~W0u CharCJaJPK![Content_Types].xmlN0EH-J@%ǎǢ|ș$زULTB l,3;rØJB+$G]7O٭V-\".B-?7}>FTlon77Ԝx?|+NST2 XiEꭶ?j]m^_^+P[wD+PW-W_YW ͶW5 R ~,VAFXuVKsKTCV]rŦZ-t  ;b6#42% qv8›q. B˟TDFLڐAB&~ ^]ُ=q==~NV;(VG:=ՃxiJ!x?>~?< }a\G5TLx?y;~nьHbwc:EGP~Ds |w<4rg!Rt|+c m߆6Il!Q] sZ;aQITjYV`<$6D='Ienb~k3MHýS^P\wr^+j~k~vt~t«(:zQk_۶sWb/?ѹS)uzݜnrʵ+Am~޽: 5ˁWrbJAүsUTjzf5η1T>汀*^PK!ѐ'theme/theme/_rels/themeManager.xml.relsM 0wooӺ&݈Э56?$Q,.aic21h:qm@RN;d`o7gK(M&$R(.1r'JЊT8V"AȻHu}|$b{P8g/]QAsم(#L[PK-![Content_Types].xmlPK-!֧6 0_rels/.relsPK-!kytheme/theme/themeManager.xmlPK-!.theme/theme/theme1.xmlPK-!ѐ' theme/theme/_rels/themeManager.xml.relsPK] t  h;=NZ[B\ "(37 p $ V @99@;;<^<<<0=|===Z ZZJZZ~[[[<\B\ !#$%&')*+,/01245689`XXT # @H 0(  0(  B S  ?_GoBack OLE_LINK5 OLE_LINK6 '' ++.2os"*@Oil(#\t|p x 3 4 6 Z ` a c  .2389;<FHLMYZbgkpqz~&+37;?CLxy  KMs3tu@W  /;LV{%Qx  I,\#|6H^H`o(.(\^(`\)\^`\.p\^p`\. \^ `\) \^ `\.\\^\`\.\^`\)\^`\.h^h`o(H\^H`\)\^`\.\^`\.4\^4`\) \^ `\.| \^| `\. \^ `\)\^`\.I,\#|6!:        ^         mDVm\4 1 T kO a[ {y K I5XO:),su+R6Oz 6hls)G} 4z" #l&Q&V&6'+(:F(}*Ie*y*7e+]m+6072rr24g67p7/8H9FR9W9"D<=e>hDFWDGzH!ISAMbM;(NBN1PPQ RaTVgtW~WPYR[I_Yadie`;fCihjE6mIoIoVTpupt{qwJx-_yz@}wK~X:\St;8Tr`Z}NO`%>X!D(:= seeduL2L`VQe7}~ilc:_T(Cp+@5k80(XZh2s0u;#3MzuKYD<:PRXW{g%&d !9K]P%Ox{S%)p p@)0b-` lF}h @M@$d  $@8:<ZUnknown G*Ax Times New Roman5Symbol3. *Cx Arial-= |8wiSO1. R<(_oŖў7.@Calibri;([SOSimSun7: SymeteoA$BCambria Math 1h;ib$'M= = ?!%),.:;>?]}    & 0 2 3 : !6"000 0 00000006:>@DZ\^ =@\]^$([{  0 0 000000Y[];[:qHX  $P2!xx  xiqiang yangUsert i Z'`IZ'  Oh+'0t  $ 0 <HT\dlxiqiang yang Normal.dotmUser21Microsoft Office Word@ @4b@0z;p"@/,R= ՜.+,D՜.+, X`lt|   8@ _PID_HLINKSAd D>http://www.cnmooc.org/portal/session/bulletin/index/2620.mooc'http://cc.sjtu.edu.cn/G2S/site/preview/home/v?currentoc=8007  !"#$%&'()*+,-./0123456789:<=>?@ABCEFGHIJKLMNOPQRSTUVWXYZ\]^_`abdefghijmRoot Entry F,RoData ; 1TableD,WordDocument8tSummaryInformation([DocumentSummaryInformation8cCompObjn  FMicrosoft Word 97-2003 ĵ MSWordDocWord.Document.89q